Research and development Analyses and services Ecotoxicology

Ecotoxicology

Contact - Doc. RNDr. Luděk Bláha, Ph.D.

Standardized aquatic / extraction tests


1. Algal growth inhibition test (72-96h)

  • EN ISO 8692
 

2.Daphnia magna acute immobilization test (24-48h)

  • EN ISO 6341
 

3. Daphnia magna chronic reproduction test (21days)

  • EN ISO 10706
 

4. Frog Embryo Teratogenicity Assay Xenopus (FETAX) (96h)

  • ASTM E 1439
 

5. Inhibition of marine bacteria Vibrio fisheri luminescence ("Microtox") (30 min)

  • EN ISO 11348
 

6. Fish early life-stage toxicity test with Danio rerio eggs (48 h)

  • ISO 15088
 

7. Inhibition of Pseudomonas putida bacterial growth (16h)

  • ISO 10712
 

Sediment contact toxicity tests


1. Sediment toxicity test with Chironomus riparius larvae (10 days)

  • Evaluation of the total number of chironomids compared to their number in the reference sediment
 

2. FLASH bacterial contact test with Vibrio fisheri (30 sec)

  • Kinetic method of luminescence inhibition / internal correction on the colored samples and suspensions
 

Genotoxicity and other specific tests


1. SOS-chromotest®

  • Bacterial genotoxicity test (E. coli)
 

2. GFP yeast assay

  • Reporter genotoxicity test on eukaryotic cells (yeast S. cerevisiae)
 

3. Dioxin toxicity test (H4IIE.luc ~ DR-CALUX) (24-72h)

  • Evaluation of the presence and the total concentration of dioxine-like active compounds (PCDD/Fs, PCBs, PAHs and their derivatives)
 

4. Xenoestrogenicity test (MVLN ~ ER-CALUX) (6-24 h)

  • Evaluation of the presence and the total effect of xenoestrogens and antiestrogens in sample
 

5. Endocrine disruption tests - androgenity, toxicity linked with the retinoic acid receptor, steroidogenesis (MDA, H295R, P19/A15)

  • Evaluation of the presence and total effect of compounds disturbing hormonal balance and acting by various mechanisms of action (endocrine disruption)
 

Soil biotests with invertebrates


1. Earthworm reproduction, mortality and growth test with Eisenia fetida

  • ISO 11268-1 (1993)
  • ISO 11268-2 (1998)
  • OECD Test No. 207 (1984)
  • OECD Test No. 222 (2000)
 

2. Bioaccumulation biotest with earthworm Eisenia fetida and Enchytraeus albidus

  • ASTM E 1676-97 (2000)
  • OECD draft (2006)
 

3. Enchytraeidae reproduction, mortality and growth test with Enchytraeus albidus a Enchytraeus crypticus

  • ISO 16387 (2004)
  • OECD Test No. 220 (2004)
 

4. Collembola reproduction and mortality test with Folsomia candida a Folsomia fimetaria

  • ISO 11267 (1999)
 

5. Caenorhabditis elegans mortality test

  • ASTM E 2172-01 (2001)
 

6. Earthworm / Enchytraeidae avoidance tests in soil and soil substrates

  • ISO 11269-1 (1993)
  • ISO 11269-2 (2005)
  • OECD Test No. 208 (1984)
  • OECD Test No. 227 (2000)
 

7. Germination and growth test on terrestrial plants (Sinapis alba, Avena sativa, Brasicca rapa etc.)

  • ISO/DIS 17512-1 (2006)
 

8. Bioavailability of compounds in soil

  • Reid et al. (2000) and others
 

Biotests on soil microorganisms


1. Biotests on soil microorganisms

  • OECD Test No. 216 (2000)
  • OECD Test No. 217 (2000)
 

2. Determination of soil microbial biomass – fumigation-extraction method

  • ISO 14240-2 (1997)
 

3. Determination of soil microbial biomass – fumigation-extraction method

  • ISO 16072 (2002)
 

4. Determination of respiration and growth kinetics of the soil microbial community

  • ISO 17155 (2002)
 

5. Determination of ammonification, nitrification and the total nitrogen mineralization

  • ISO 14238 (1997)
  • ISO 15685 (2004)
 

6. Determination of the dehydrogenase activity

  • ISO 23753-1 (2005))
 

7. Determination of the function diversity of soil microorganisms – BIOLOG method

  • Kandeler et al. (1996)