RECETOX Research Infrastructure Equipment and Services

Equipment and Services of RECETOX Research Infrastructure

Instrumentation of the RECETOX Research Infrastructure is distributed between the three units (trace analytical laboratories, GENASIS information system and ELSPAC database) as well as in four individual research programmes.

Laboratory infrastructure and instrumentation available in RECETOX’s three core facilities

Trace Analytical Laboratories

  • High volume and low volume active sampling devices for gas and particle phases
    of atmosphere (Digitel, Leckel)
  • Cascade impactors for size-stratification of atmospheric particles
  • Wet deposition samplers
  • Passive air and water sampling devices
  • Analyzers of total organic carbon and elementary carbon
  • High resolution mass spectroscopy Waters Autospec Premier equipped with Agilent 7890 gas chromatography
  • High resolution mass spectroscopy Thermo Scientific DFS equipped with two Thermo HRGC trace 1310 gas chromatographic systems
  • High resolution mass spectroscopy Agilent qTOF 6550 with Agilent 1290 liquid chromatography and Agilent 7890 gas chromatography
  • Inductively Coupled Plasma Mass Spectroscopy and gas or liquid chromatography (Agilent ICP-MS 7500ce, Agilent ICP-MS 7700, Agilent HRGC 6890, Agilent HPLC 1100)
  • Mass spectroscopy (AB-SCIEX MS-MS QTRAP 5500 combined to liquid chromatography Agilent HPLC 1100
  • Mass spectroscopy MS-MS XEVO TQ-S combined to liquid chromatography Waters UPLC Acquity
  • Mass spectroscopy APGC MS-MS XEVO TQ-S combined to gas chromatography Agilent 7890
  • Mass spectroscopy Agilent QQQ 7000B combined to liquid chromatography Agilent 7890, 2 pc
  • Mass spectroscopy Waters MS-MS Quattro micro GC combined to gas chromatography Agilent 6890
  • Preparatory liquid chromatography (Waters) and others

GENASIS Information System

  • Development of data repositories, interdisciplinary multilevel database systems and web portals optimized for multiuser groups
  • Comprehensive on-line information systems for management of environmental data
  • Development of environmental models, assessment systems and tools supporting decision-making in ecological risk assessment
  • Data management and visualization tools using GIS
  • Analyses of causality, spatial patterns and temporal dynamics in environmental data
  • Scientifically-oriented software development and advanced expertise in statistical data analyses
  • Information and communication technologies and environmental informatics with focus on development of novel tools describing complexity of the environment

ELSPAC database

  • The ELSPAC cohort can be used as a control group for a number of medical analyses
  • Given its regional coverage, the ELSPAC study extensively monitors the Brno region and provides data on inhabitants’ migration or children’s movement between education facilities in the region
  • Given the duration of the ELSPAC study, it can be used as a basis to investigate the impact of the political and social transition from a communist to democratic regime and market economy on human health and quality of life
  • Family environment has been extensively monitored in the ELSPAC database, which can serve as a starting point to investigate the effects of the family on later successes and failures of young people
  • Based on a detailed family medical history, the health status of the investigated cohort can be assessed from the viewpoint of congenital and acquired health risks
  • Records of school results and child behaviour can bring new insight to education issues with a comprehensive view on the child in the context of his/her health status and family environment
  • Development of a comprehensive on-line browser and reporting system working with the database of the Czech ELSPAC cohort

Laboratory infrastructure and instrumentation available in RECETOX’s four scientific programmes

Environmental Chemistry and Modelling

  • Volatilization chambers
  • Sorption columns
  • Cold rooms
  • Solar simulator
  • Dermal exposure assessment system - Franz diffusion cell in vitro

Organic Photochemistry and Supramolecular Chemistry

  • Nanosecond laser flash spectroscopy (500 ps Nd-YAG laser)
  • Pump-and-probe spectroscopy (150 fs Ti: sapphire laser)
  • Optical benches for fast transient kinetic measurements
  • UV/VIS/NIR absorption spectroscopy (transmission and reflectance measurements)
  • Spectrofluorimeter (excitation and emission spectra)
  • Optical benches for steady-state irradiation experiments
  • Laboratory of organic synthesis
  • Walk-in cold room and photochemical cryogenic photoreactors
  • NMR, GC, HPLC, GC-MS, HPLC-TOF, MALDI, and electrochemical instrumentation (shared)

Protein Engineering

  • Stopped Flow a Rapid Quench Flow (Biologic)
  • CD Spectrophotometer (Applied Photophysics)
  • Microcalorimeter ITC and DSC (Microcal)

Ecotoxicology

  • Laboratory organisms for ecotoxicology and toxicology (D. rerio, X. laevis, D. magna, Ch. riparius, C. elegans, E. fetida, F. candida, E. crypticus, algae, cyanobacteria, bacteria)
  • Set of ZEISS microscopes (Axio Imager, Axio Observer Z1)
  • Flow cytometer equipped with photographic recording (FlowCAM)
  • Microbial respiration assessment system (OxiTop)
  • GC-TCD on-line determination of gas concentrations (CO2, O2, CH4, alkans)
  • Laboratory of cell and molecular toxicology