RECETOX Research Infrastructure Equipment and services

Equipment and services of RECETOX Research Infrastructure

Technical and human capacities of three RECETOX RI core facilities provide support for interdisciplinary studies quite unique in this area. Complemented with technology and expertise available in the research programmes of the RECETOX Centre they offer a broad expertise (environmental, atmospheric and analytical chemistry, photochemistry, supramolecular and green chemistry, biochemistry, protein engineering, biosensors and biocatalysis, enzymology, microbiology, molecular biology and genetics, ecology and pedology, toxicology and ecotoxicology, environmental impact, human exposure and risk assessments, pathophysiology, environmental and pharmacokinetic modelling, biostatistics and data analysis, development of software, databases and web portals, information and communication technologies) necessary for addressing the most relevant national and European research priorities and societal challenges in the cross-cutting area of Environment and Health. More than 100 high-end instruments covering all RECETOX research areas.

The list of the instrumentation and services

Centralized in core facilities

 

Instrument

TRACE
ANALYTICAL
LABORATORIES

High volume, medium and low volume active sampling devices for gas and particle phases of atmosphere (Digitel, Leckel)

Cascade impactors for size-stratification of atmospheric particles

Particles samplers

Wet deposition samplers (Baghirra, WS 1m, WS 314 GI)

Passive air and passive water sampling devices (Wildco)

Analyzers of total organic carbon and elementary carbon (TOC, EC/OC)

High resolution mass spectroscopy Waters Autospec Premier equipped with Agilent 7890 gas chromatography

High resolution mass spectroscopy Thermo Scientific DFS equipped with two Thermo HRGC trace 1310 gas chromatographic systems

High resolution mass spectroscopy Agilent qTOF 6550 with Agilent 1290 liquid chromatography and Agilent 7890 gas chromatography

Inductively Coupled Plasma Mass Spectroscopy and gas or liquid chromatography (Agilent ICP-MS 7500ce, Agilent ICP-MS 7700, Agilent HRGC 6890, Agilent HPLC 1100)

Mass spectroscopy (AB-SCIEX MS-MS QTRAP 5500 combined to liquid chromatography Agilent HPLC 1100

Mass spectroscopy APGC MS-MS XEVO TQ-S combined to gas chromatography Agilent 7890

Mass spectroscopy Agilent QQQ 7000B combined to liquid chromatography Agilent 7890, 2 pc

Mass spectroscopy Waters MS-MS Quattro micro GC combined to gas chromatography Agilent 6890

Preparatory liquid chromatography and GPC system

Mass spectroscopy combined to gas chromatography GC/MS Thermo Scientific Q Exactive GC Orbitrap

Mass spectroscopy combined to liquid chromatography Thermo Scientific Orbitrap Fusion

Mass spectroscopy combined to liquid chromatography UPLC 1290 Infinity II/MS-MS Triple Quadrupole 6495 (Agilent)

 

 

Expertise

TRACE
ANALYTICAL
LABORATORIES

  • Sampling tools for assessment of distribution and behavior of chemical compounds in the environment (active/passive samplers)
  • Analyses of matrices (air, soil, sediments, water, biota) for polychlorinated biphenyls, organochlorine pesticides, polycyclic aromatic hydrocarbons, polychlorinated dibenzo-p-dioxines and furanes, brominated and organophospate flame retardants, perflorinated compounds, and others (sweeteners, pharmaceuticals, personal care products)
  • Analyses of matrices (soil, sediments, water, biota) for heavy metals, organometallic compounds
  • Elementar analyses of various samples and analyses of toxic heavy metals in abiotic and biotic matrices
  • Speciation analyses of toxic and essential elements in the environment and biota
  • Laboratory studies of environmental processes (volatilization, sorption, phase equilibria and inter-phase transport, and degradation)
  • Field studies of environmental distribution and transport
  • Modelling of environmental distribution and transport
  • Indoor exposure and biomonitoring
  • Human exposure assessment
  • Human health risk assessment and modelling

POPULATION
STUDIES

  • The ELSPAC database cohort can be used as a control group for a number of medical analyses.
  • Due to its territorial character, the ELSPAC study extensively monitors the Brno region and provides data on inhabitants’ migration or children’s fluctuation between education facilities in the region
  • Due to the duration of the ELSPAC study, it can be used as a basis to investigate the impact of the political and social transition from a communist to democratic regime and market economy on human health and quality of life.
  • Family environment has been extensively monitored in the ELSPAC database, which can serve as a starting point to investigate effects of the family on later successes and failures of young people.
  • Based on a detailed family medical history, health status of the investigated cohort can be assessed from the viewpoint of congenital and acquired health risks.
  • Records of school results and behavior of children can bring new insight to education issues with a comprehensive view on the child in the context of his/her health status and family environment.
  • Development of a comprehensive on-line browser and reporting system working with the database of the Czech ELSPAC cohort.

DATABASE AND
INFORMATION
SYSTEM

  • Development of data repositories, interdisciplinary multilevel database systems and web portals optimized for multiuser groups
  • Comprehensive on-line information systems for management of environmental data
  • Development of environmental models, assessment systems and tools supporting decision-making in ecological risk assessment
  • Data management and visualization tools using GIS
  • Analyses of causality, spatial patterns and temporal dynamics in environmental data
  • Scientifically oriented software development and advanced expertise in statistical data analyses
  • Information and communication technologies and environmental informatics with focus on development of novel tools describing complexity of the environment.

 

Centralized in research divisions

 

Instrument

Expertise

ORGANIC
PHOTOCHEMISTRY
AND
SUPRAMOLECULAR
CHEMISTRY
LABORATORY

Nanosecond laser flash spectroscopy system (170 ps Nd-YAG laser)

Understanding the Mechanisms of Photochemical Reactions

Pump-and-probe spectroscopy system (150 fs Ti:sapphire laser)

Photochemistry of Organic Compounds in Ice and Snow

Fast transient kinetic measurements under cryogenic conditions

Environmental Photochemistry

UV/VIS/NIR absorption spectroscopy (transmission and reflectance measurements)

Design of New Photoactivatable Compounds

Spectrofluorimeter (excitation and emission spectra)

Design, synthesis and characterization of new supramolecular assemblies

Optical benches for steady-state irradiation experiments

 

Laboratory of organic synthesis

Walk-in cold room and photochemical cryogenic photoreactors

NMR, GC, HPLC, GC-(HR)MS, HPLC-TOF, MALDI (shared)

PROTEIN
ENGINEERING
LABORATORY

Advanced Compact Ion Chromatograph

Design of biosensors for detection of environmental pollutants

CD Spectropolarimeter (2x)

Determination of the structures of protein in a solution

Colony picking robot

Picking of colonies carrying mutant proteins during directed evolution experiments.

Desintegrator

Desintegration of bacterial cells overproducing engineered proteins

Differential scanning calorimeter

Determination of thermodynamic parameters and stabilities of proteins

Fermenter (2x)

Production of natural and engineered biocatalysts for degradation of toxic wastes

Fluorescence spectrophotometer

Analysis of enzymatic activities and substrate specificity profiles

FPLC (3x)

Purification of intracellular proteins using liquid chromatography

Gas Chromatograph (5x)

Determination of very low concentrations of volatile compounds

Isothermal titration calorimeter (2x)

Analysis of protein-ligand interactions

Mass Spectrometer (2x)

Identification of reaction products and toxic intermediates

Microfluidizer

Formation of emulsions for enzymatic catalytic reactions

Microplate Reader (2x)

Screening of substrate specificities and screening of inhibitors

Nanodrop

Determination of protein and DNA concentrations

pH Titrator

Determination of pH in liquid media

Pipetting robot

Automated handling of small of volumes of liquids

Rapid Quench Flow (2x)

Analysis of transient kinetics for determination of rate-limiting steps

Spectrophotometer Ultrospec

Screening of enzymatic reactions

Stopped Flow (2x)

Analysis of transient kinetics for determination of rate-limiting steps

ECOTOXICOLOGY

Laboratory organisms for ecotoxicology and toxicology (D. rerio, X.laevis, D.magna, Ch.riparius, C.elegans, E.fetida, F.candida, E.crypticus, algae, cyanobacteria, bacteria)

In vivo ecotoxicology profiling with a set of bioassays; including both aquatic media and contact toxicity testing of solid samples

Cell and molecular toxicology lab with robotics (epMotion), real-time monitoring (xCelligence), advanced 3D testing (organoids), and high-end ZEISS microscopes (Axio Imager, 2x Axio Observer Z1)

In vitro toxicity assessment, predictions of long-term impacts of chemicals and natural toxins on both human health and organisms

High content analyses bz flow through cytometry with photographic recording FlowCAM and automated high throughput scanning (TissueFAXS)

Biochemical mechanisms and markers of stress and specific modes of toxicity

Microbial respiration assessment system (OxiTop) and GC-TCD on-line determination of gas concentrations (CO2, O2, CH4, alkanes)

Tools for effective assessment of chemical bioaccumulation and effects in contaminated water, sediments, soil and air

Cell- and yeast- based bioreporter detection tools for AhR, ER, AR, GR, RAR/XR, ThR, PPAR and other molecular targets

Risks of dioxin-like compounds, genotoxic and endocrine disrupting chemicals in the environment