Structure Protein engineering

Protein engineering

More information:

  • Personnel
  • Publications
environmentalni chemie

This research programme focuses on fundamental principles of enzymatic catalysis and the development of protein and cellular biocatalysts for environmental, chemical and biomedical applications. Protein engineering and synthetic biology techniques are used to produce unique biocatalysts, which are isolated from bacteria colonising extreme environments. New methodological concepts and software tools are being developed for rational design of proteins. The speciality of the group is the integration of theoretical and experimental approaches through close collaboration of three research teams: the model, genetic and kinetic research teams.

The group has published more than 100 original articles (41 in the last 5 years) in high impact journals, 10 (4) book chapters and 3 (1) patents for the use of enzymes in decontamination and biocatalysis. The group has founded the first biotechnology spin-off of Masaryk University, Enantis Ltd., and co-founded the software spin-off Caver-Soft Ltd. The scientific excellence of the team has been recognized by an award from the European Molecular Biology Organisation and the Howard Hughes Medical Institute.

Research Topics

  • Biosensors for detection of environmental pollutants
  • Computational tools (Caver, Hotspot Wizard) for designing biocatalysts
  • Computer-assisted screening of substrates and inhibitors
  • Engineered biocatalysts for “green” production for valuable chemicals
  • Microfluidics for ultra-fast screening and enzyme kinetics
  • Natural and engineered biocatalysts for degradation of toxic wastes

Research Infrastructure

  • Stopped Flow a Rapid Quench Flow (Biologic)
  • CD Spectrophotometer (Applied Photophysics)
  • Microcalorimeter ITC and DSC (Microcal)