Negi attained his Bachelor of Pharmacy from University Institute of Pharmaceutical Sciences (UIPS), Panjab University, India, and later obtained his Master of Science degree in Regulatory Toxicology from National Institute of Pharmaceutical Education and Research (NIPER), Mohali, India. His master's thesis was focused on investigating the effects of dietary activation of the master regulator of endogenous antioxidants; Nrf2, in diabetes and related complications in laboratory animals. During his post-graduation studies, he worked independently in the Facility for Risk Assessment and Intervention Studies under the supervision of Dr G. B. Jena where he gained research experience in laboratory techniques, including theoretical and practical knowledge of various challenging courses.

Following his post-graduation, Negi worked as a drug regulatory affairs associate in a private sector pharmaceutical industry and a multinational consulting firm. Acting as a liaison between the organizations and federal agencies, he was mainly responsible for authoring and submitting critical regulatory documents including investigational new drug and clinical trial applications to the pharmaceutical regulatory agencies, intending to secure approval or marketing authorization.

Since August 2020 Negi is employed as a PhD ESR at the RECETOX in Dr Blaha's group. His research focuses on the characterization of ecotoxicological effects and mechanism of action of novel flame retardants in zebrafish and in vitro models. In this work, various molecular initiating events, and key events associated with flame retardants-mediated reproductive and metabolic dysfunction will be characterized through high through-put screening and toxicogenomic approach.