ESR 6: Diana Ilyaskina


Country of origin: Russia

Host institution: Vrije Universiteit Amsterdam

Contact: d.ilyaskina@vu.nl

About me

Studying in the city surrounded by the Japanese Sea, Diana has developed a strong interest in marine biology, ecology, and biochemistry. Diana graduated with a Bachelor and Master of Biology with the majors “Molecular Cell Systems and Biotechnology” and “Biological systems: structure, function, technology” in the Far Eastern University, Vladivostok, Russia. Diana conducted several projects related to the investigation of properties of bioactive proteins from mollusks and several ecological projects aimed to discover the influence of the seasonal changes and pollution on biology marine organisms. During her previous work, Diana mastered different methods in biochemistry, cytology, molecular biology, microbiology, and statistics.

Among the main thesis projects, Diana also had the opportunity to gain teaching experience and organize the research projects for young students and scholars, which aimed to develop their attitude and skills in tumor biology and biochemistry.

Currently, Diana is enrolled as a PhD student in the department Environment & Health of the Faculty of Science of the Vrije Universiteit Amsterdam.

Her research within PRORISK focuses on metabolomics to investigate the influence of toxic chemicals on metabolic pathways and link it with phenotype changes. During the project, Diana is going to work with terrestrial and aquatic arthropods.

Project

Project title: Linking phenotype, developmental and behavioral outcomes to metabolomic pathways in aquatic snails and terrestrial arthropods
Objectives:

To develop a combined in vivo and in vitro metabolomic approach to investigate molecular mechanisms and pathways of neonicotinoid toxicity.
Establish/evaluate AOP for neurodevelopmental and behavioural effects of neonicotinoids on Lymnaea stagnalis (aquatic) and Folsomia candida (soil) by linking involved metabolic pathways with phenotypic/developmental/behavioural changes.
To link tissue specific exposure information to molecular information at the anatomical level.

Expected results:

New insights into molecular mechanisms and pathways of neonicotinoid toxicity. In vitro and combined cross-omic strategy (metabolomics, proteomics and genomics; collaboration with ESR5) to confirm MoA and toxicity mechanism associated with the AOP.
Anchoring of metabolic profiles with pathways observed in other in vitro (ESR1, ESR2, ESR3, ESR4) and in vivo assays.
Anchoring of the chemical exposure, molecular tissue distribution and organism health profiles to MIE, KE and AO of selected AOPs.
Revised conceptual AOP for neurodevelopmental disorders and final version submitted to the OECD-EAMST for expert evaluation.

Place: Amsterdam, Netherlands
Planned secondments:
MU, M22, 2 months, in vitro studies
RIWA, M26, 2 months, data analysis
Work packages: 3 - Linking molecular responses to effects at higher biological levels using adverse outcome pathways (AOPs)
4 - Predicting effects on ecosystem services through AOPs
Supervisors:

Marja Lamoree, VUA
Pim Leonards, VUA

Host institution and enrolment: Vrije Universiteit Amsterdam