Sequence data processing and analysis

This bioinformatics core facility provides advanced computational resources and expertise to support biological research projects. We offer a wide range of bioinformatics analysis pipelines to analyze complex biological data and gain insights into biological systems. Our bioinformatics experts work closely with researchers to help them design experiments, analyze data, and interpret results.

The facility uses computational resources of high-performance computing clusters of National Grid Infrastructure operated by MetaCentrum as a part of the pan-European infrastructure built in the framework of the EGI project. We collaborate closely with Biostatistics and data analysis core facility to offer fully integrated services following the experimental life cycle from complex experimental design to data interpretation and new hypothesis generation. Our facility participates in national and European projects.

Services

The facility provides following services:

Analyses / Access to Data

  • Our services cover state-of-the-art development of pipelines and analysis of data from a wide range of omics experiments from microarrays and NGS sequencing, and include genome assembly, metagenomics (WMGS and targeted – 16SrRNA, ITS, …), phylogenetics, genome analysis (targeted panels of mutations, WGS, WES, SNP calling), transcriptome analysis (RNAseq, miRNAseq, expression microarrays), epigenomics (epigenetic chips).
  • All the data are subjected to strict quality control and to multiple profiling computational pipelines to obtain complementary features sets for secondary, statistical, and machine learning analysis. The methods combine out in-development approaches and the state-of-the-art tools for quality control, genome assembly, taxonomic profiling, functional profiling and profiling of metabolic potential, estimation of microbiome responsible for changes metabolic profiles, estimation of crosslink between xenobiotics, host metabolism and gut microbiome (internal tool in development); variant calling, genotype calling, differential gene expression analysis, …etc.
  • Additionally, we provide training and support for researchers who are new to bioinformatics or who need help with specific tools or analyses.

Consultations

Whether you are just thinking about a new experiment or already have the resulting data, you may contact us with your questions and we will assess the feasibility of the various analyses required. Ideally, you would contact us at the experimental design stage, so we can ensure that all the relevant information will be available to answer your questions.

We also provide consultations on data analysis and methods.

Access

Access to the laboratories is available to academic users and researchers, including representatives of the commercial sphere and industry. Academic users and researchers can access through the Open Access project. Application for access is made at: https://openaccess.recetox.cz/. Industrial users can directly contact coordinator of RECETOX REsearch Infrastructure RNDr. Petra Růžičková, Ph.D. (openaccess@recetox.muni.cz), who will answer questions about available capacities, expertise and other conditions of cooperation.

Contact

For more or specific information, please, contact:

Mgr. Eva Budinská, Ph.D.

Head of the Biostatistics and Bioinformatics in Exposomics Facility

phone: +420 549 49 3993
e‑mail:
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Mgr. Eva Budinská, Ph.D.

Head, Integrative Bioinformatics and Biostatistics

phone: +420 549 49 3993
e‑mail:

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